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SELECTED PUBLICATIONS (in reverse chronological order, representative of 80 papers, 11 book chapters, 124 abstracts, 6 patents/patent applications)

Guillemette, M.D., Park, H., Hsiao, J.C., Jain, S.R., Larson, B.L., Langer, R., Freed, L.E., Combined Technologies for Microfabricating Elastomeric Cardiac Tissue Engineering Scaffolds, Macromolecular Bioscience DOI: 10.1002/mabi.201000165, 2010 (in press)

Abrahamsson CK, Yang F, Park H, Brunger JM, Valonen PK, Langer R, Welter JF, Caplan AI, Guilak F, Freed LE. Chondrogenesis and Mineralization During In Vitro Culture of Human Mesenchymal Stem Cells on Three-Dimensional Woven Scaffolds. Tissue Eng Part A. 2010 Sep 6. [Epub ahead of print] PubMed PMID: 20673022.

Valonen PK, Moutos FT, Kusanagi A, Moretti MG, Diekman BO, Welter JF, Caplan AI, Guilak F, Freed LE. In vitro generation of mechanically functional cartilage grafts based on adult human stem cells and 3D-woven poly(epsilon-caprolactone) scaffolds. Biomaterials. 2010 Mar;31(8):2193-200. Epub 2010 Jan 19. PubMed PMID: 20034665; PubMed Central PMCID: PMC2824534.

Mobine HR, Engelmayr GC Jr, Moussazadeh N, Anwar TR, Freed LE, Edelman ER. Encapsulated pheochromocytoma cells secrete potent noncatecholamine factors. Tissue Eng Part A. 2009 Jul;15(7):1719-28. PubMed PMID: 19125641; PubMed Central PMCID: PMC2792103.

Freed, L.E., Engelmayr, G.C., Borenstein, J.T., Moutos, F.T., Guilak, F. (2009): Advanced material strategies for tissue engineering scaffolds. Advanced Materials 21(32-33): 3410-3418 (inside cover of issue)

Cheng, M.Y., Moretti, M., Engelmayr, G.C., Freed, L.E. (2009): Insulin-like growth factor-I and slow, bi-directional perfusion enhance the formation of tissue-engineered cardiac grafts. Tissue Engineering, 15(3): 645-653

Engelmayr, G.C., Cheng, M., Bettinger, C.J., Borenstein, J.T., Langer, R., Freed, L.E. (2008): Accordion-like honeycombs for tissue engineering of cardiac anisotropy. Nature Materials, 7: 1003-1010 (cover of issue, with commentary on pp. 932-933)

Radisic M., Park, H., Martens, T.P., Salazar-Lazaro, J.E., Geng, W., Wang, Y., Langer, R., Freed, L.E., Vunjak-Novakovic, G. (2008): Pre-treatment of synthetic elastomeric scaffolds by cardiac fibroblasts improves engineered heart tissue. J Biomedical Materials Research-A 86: 713-724

Nichol, J. W., Engelmayr, G. C., Cheng, M., and Freed, L. E. (2008): Co-culture induces alignment in engineered cardiac constructs via MMP-2 expression. Biochemical Biophysical Research Comm 373: 360-365

Augst, A., Marolt, D., Freed, L.E. , Vepari, C., Meinel, L., Farley, M., Fajardo, R., Patel, N., Gray, M.L., Kaplan, D.L., Gordana Vunjak-Novakovic, G. (2008): Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human bone marrow stem cells, silk scaffolds and bioreactors. J of the Royal Society Interface 5: 929-939

Freed, L.E., Guilak, F. (2007): “Engineering functional tissues.” In: Principles of Tissue Engineering 3rd edition, Ch.11 (R.P. Lanza, R. Langer and J. Vacanti, eds., Academic Press). Ch. 11, pp. 137-153.

Moutos, F.T., Freed, L.E., Guilak, F. (2007): A biomimetic three-dimensional woven composite scaffold for functional tissue engineering of cartilage. Nature Materials 6:162-167 (cover of issue, with commentary pp. 89-90).

Cheng, M. Y., Park, H., Engelmayr, G. C., Moretti, M., and Freed, L. E. (2007): Effects of regulatory factors on engineered cardiac tissue in vitro. Tissue Engineering 13: 2709-2719

Freed, L.E., Guilak, F., Guo, X.E., Gray, M.L., Tranquillo, R., Holmes, J., Radisic, M., Sefton, M., Kaplan, D., Vunjak-Novakovic, G. (2006): Advanced tools: scaffolds, bioreactors, and signaling, Tissue Engineering 12: 3285- 3305 (cover of issue)

Marolt, D., Augst, A., Freed, L.E., Vepari, C., Fajardo, R., Patel, N., Gray, M., Farley, M., Kaplan, D., Vunjak-Novakovic, G.  (2006): Bone and cartilage tissue constructs grown using human bone marrow stromal cells, silk scaffolds, and rotating bioreactors. Biomaterials 27(36):6138-6149

Radisic, M, Park, H, Chen, F, Wang, Y, Dennis, R, Langer, R, Freed, LE , Vunjak-Novakovic, G. (2006): Biomimetic approach to cardiac tissue engineering: oxygen carriers and channeled scaffolds. Tissue Engineering, 12(8): 1-15 (cover of issue)

Boublik J., Park, H., Radisic, M., Tognana, E., Chen, F., Pei , M., Vunjak-Novakovic, G., Freed, L.E. (2005): Mechanical properties and remodeling of hybrid cardiac constructs made from heart cells, fibrin, and a biodegradable elastomeric knitted fabric. Tissue Engineering 11 (7-8): 1122-1132

Tognana, E., Padera, R.F., Chen, F., Vunjak-Novakovic, G., Freed, L.E. (2005): Development and remodeling of engineered cartilage-explant composites in vitro and in vivo. Osteoarthritis and Cartilage 13(10): 896-905

Tognana, E., Chen, F., Padera, R.F., Leddy, H.A., Christensen, S.E., Guilak, F., Vunjak-Novakovic, G., Freed, L.E. (2005): Adjacent tissue (cartilage, bone) affect the functional integration of engineered calf cartilage in vitro. Osteoarthritis and Cartilage 13(2): 129-138

Schäfer, D., Seidel, J.O, Martin, I., Jundt, G., Heberer, M., Grodzinsky, A.L., Vunjak-Novakovic, G., Freed, L.E. (2004): Engineering and characterization of functional osteochondral repair tissue. Orthopäde 33: 721–726

Radisic, M., Park, H. Shin, H., Consi, T., Schoen, F.J., Freed, L.E., Vunjak-Novakovic, G. Novakovic G. (2004): Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffolds. Proc. National Acad of Sciences USA 101: 18129-18134.

Radisic, M., Yang, L, Boublik, J, Cohen, R.J., Langer, R., Freed, L.E., Vunjak-Novakovic, G. (2004): Medium perfusion enables engineering of compact and contractile cardiac tissue. Am J Physiology (Heart & Circulatory Physiol.) 286: H507-516.

Bursac, N., Papadaki, M., White, J.A., Eisenberg S.R., Vunjak-Novakovic, G., Freed, L.E. (2003): Cultivation in rotating bioreactors promotes maintenance of cardiac myocyte electrophysiology and molecular properties, Tissue Engineering, 9(6): 1243-1253

Freed, L.E., Rupnick, M.A., Schaefer, D., Vunjak-Novakovic, G. (2003): “Engineering functional cartilage and cardiac tissue: in vitro culture parameters”. In: Functional Tissue Engineering: the Role of Biomechanics (F. Guilak, D. Butler, D. Mooney, S. Goldstein, eds, Springer Verlag) Ch. 27, pp. 360-376.

Seidel, J.O., Pei, M., Gray, M.L., Langer, R, Freed, L.E., Vunjak-Novakovic, G. (2004): Long term culture of tissue engineered cartilage in a perfused chamber with mechanical stimulation. Biorheology 41: 445-458

Freed, L.E . and G. Vunjak-Novakovic, (2002) “Cell-Polymer-Bioreactor Systems”. In: Methods of Tissue Engineering (A. Atala and R.P. Lanza, eds. Academic Press) Ch. 6, pp. 99-114.

Freed, L.E. and G. Vunjak-Novakovic (2002) “Spaceflight bioreactor studies of cells and tissues”. In: Cell Biology and Biotechnology in Space ( A. Cogoli, ed., Elsevier Science) Advances in Space Biology and Medicine Ch. 8, pp. 177-195.

Vunjak-Novakovic, G., Searby, N.D. de Luis, J., Freed, L.E. (2002): Microgravity studies of cells and tissues. Ann. N.Y. Acad. Sci. 974: 504-517

Schaefer, D., Martin, I., Jundt, G., Seidel, J., Heberer, M., Grodzinsky, A.J, Bergin, I., Vunjak-Novakovic, G., Freed, L.E. (2002): Tissue engineered composites for the repair of large osteochondral defects. Arthritis and Rheumatism, 46: 2524-2534

Pei, M., Solchaga, L.A., Seidel, J., Zeng, L, Vunjak-Novakovic, G., Caplan, A.I., Freed, L.E. (2002): Bioreactors mediate the effectiveness of tissue engineering scaffolds, FASEB J. 16: 1691-1694

Papadaki, M., Bursac, N., Langer, R., Merok, J., Vunjak-Novakovic, G., Freed, L.E. (2001): Tissue engineering of functional cardiac muscle: molecular, structural and electrophysiological studies, Am J Physiology (Heart & Circulatory Physiol.) 280: H168–H178, 2001 (cover of issue)

Martin, I., Shastri, V.P., Padera, R.F., Yang, J., McKay, A.J., Langer, R., Vunjak Novakovic, G., Freed, L.E. (2001): Selective differentiation of bone marrow stromal cells cultured on three dimensional polymer foams J. Biomedical Materials Research  55: 229-235

Gooch, K.J., Blunk, T., Courter, D.L., Sieminski, A.L., Bursac, P.M., Vunjak-Novakovic, G, Freed, L.E. (2001): IGF-I and mechanical environment interact to modulate engineered cartilage development, Biochem. Biophys. Res. Comm., 286: 909-915

Schaefer, D., Martin, I., Shastri, P., Padera, R.F., Langer, R., Freed, L.E., Vunjak-Novakovic, G. (2000): In vitro generation of osteochondral composites.  Biomaterials  21: 2599-2606

Freed, L.E . and Vunjak-Novakovic, G. (2000). “Tissue Engineering of Cartilage”. In: The Biomedical Engineering Handbook, 2nd edition, (J.D. Bronzino, ed., CRC Press) Vol. II, pp. 124-1 – 124-26.

Freed, L.E . and Vunjak-Novakovic, G. (2000). “Tissue Engineering Bioreactors”. In: Principles of Tissue Engineering, 2 nd edition (R.P. Lanza, R. Langer and J. Vacanti, eds., Academic Press) Ch. 13, pp. 143-156.

Bursac N., Papadaki M., Cohen R.J., Schoen F.J., Eisenberg S., Carrier R., Vunjak-Novakovic G., Freed, L.E. (1999): Cardiac muscle tissue engineering: toward an in vitro model for electrophysiological studies, Am. J Physiol (Heart & Circulatory Physiology 46) 277: H433-H444

Freed L.E., Pellis N., Searby N., de Luis J., Preda C., Bordonaro J., Vunjak-Novakovic G. (1999) Microgravity Cultivation of Cells and Tissues. Gravitational and Space Biology Bulletin 12: 57-66.

Martin, I., Vunjak-Novakovic, G., Yang, J., Langer, R., Freed, L.E. (1999): Mammalian chondrocytes expanded in the presence of fibroblast growth factor-2 maintain the ability to differentiate and regenerate three-dimensional cartilaginous tissue. Experimental Cell Research 253: 681 - 688

Obradovic B, Carrier R.L., Vunjak-Novakovic G.V., Freed, L.E. (1999): Gas exchange is essential for bioreactor cultivation of tissue engineered cartilage, Biotechnology&  Bioengineering, 63:197-205

Vunjak-Novakovic, G., Martin, I., Obradovic, B., Treppo S., Grodzinsky, A.J., Langer R., Freed, L.E. (1999): Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue engineered cartilage. Journal of Orthopedic Research 17, 130-138

Martin, I, Padera, R.F., Vunjak-Novakovic, G., Freed, L.E. (1998): In vitro differentiation of chick embryo bone marrow stromal cells into cartilaginous and bone-like tissues, J. Orthopaedic Research 16: 181-189

Freed, L.E., Hollander, A.P., Martin, I., Barry, J.R., Martin, I., Vunjak-Novakovic, G. (1998): Chondrogenesis in a cell-polymer-bioreactor system, Experimental Cell Research 240: 58-65

Vunjak-Novakovic G., Obradovic B., Bursac P., Martin, I., Langer R., Freed L.E. (1998): Dynamic seeding of polymer scaffolds for cartilage tissue engineering, Biotechnology Progress 14: 193-202.

Freed, L.E., Langer, R., Martin, I., Pellis, N., Vunjak-Novakovic, G. (1997): Tissue engineering of cartilage in space, Proceedings of the National Academy of Sciences USA, 94: 13885-13890 (with commentary, pp. 13380-13382)

Freed, L.E., Vunjak-Novakovic, G (1997). Microgravity Tissue Engineering, In Vitro Cellular and Developmental Biology 33: 381-385.

Vunjak-Novakovic, G., Freed, L.E., Biron R., Langer, R. (1996). Effects of mixing on tissue engineered cartilage, Journal of the American Institute of Chemical Engineers 42 (3) 850-860.

Langer, R., Vacanti, J.P., Vacanti, C., Atala, A., Freed, L.E., Vunjak-Novakovic, G. (1995): Tissue engineering: biomedical applications, Tissue Engineering 1: 151-161.

Freed L.E., Vunjak-Novakovic, G (1995). Cultivation of Cell-Polymer Constructs in Simulated Microgravity, Biotechnology and Bioengineering 46: 306-313.

Freed, L.E., Grande, D.A., Emmanual, J., Marquis, J.C., Lingbin, Z., Langer, R. (1994): Joint resurfacing using allograft chondrocytes and synthetic biodegradable polymer scaffolds, J. Biomedical Materials Research, 28: 891-899.

Freed, L.E., Vunjak-Novakovic, G., Biron, R.J., Eagles, D., Lesnoy, D., Barlow, S.K., Langer, R. (1994): Biodegradable polymer scaffolds for tissue engineering, Nature Biotechnology 12: 689-693.

Freed, L.E., Vunjak-Novakovic G., Langer, R. (1993) Cultivation of cell-polymer cartilage implants in bioreactors, J. Cell Biochemistry 51, 257-264

Freed, L.E., G. Vunjak-Novakovic, P.A. Drinker and R. Langer. (1993): A bioreactor based on suspended particles of immobilized enzyme, Ann. Biomedical Engineering 21, 57-65

Freed, L.E., Marquis, J., Nohria, A., Mikos, A.G., Emmanual, J., Langer, R. (1993): Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers, J Biomedical Materials Research 27:11-23 (cover of issue)

Freed, L.E., Vunjak-Novakovic, G., Bernstein, H., Cooney C.L., Langer, R. The Kinetics of Immobilized Heparinase in Human Blood, Annals of Biomedical Engineering 21, 67-76, 1993

Freed, L.E. , G. Vunjak-Novakovic, P.A. Drinker and R. Langer. (1993): A Bioreactor Based on Suspended Particles of Immobilized Enzyme, Annals of Biomedical Engineering 21, 57-65

Freed, L.E., Endemann, G., Tomera, J.F., Gavino, V.C., Brunengraber, H. (1988): Lipogenesis from Ketone Bodies in Perfused Livers of Streptozotocin Diabetic Rats, Diabetes 37: 50-55

Kopito, R., Weinstock, S., Freed, L.E., Murray, D., Brunengraber, H. (1982): Metabolism of Plasma Mevalonate in Rats and Humans, Journal of Lipid Research 23: 577-583

Freed, L.E., Freed, C., O'Donnell, R. (1982): Small Signal Gain Saturation Intensity of I & II Vibrational Band Transitions in Sealed-off CO2 Lasers, Journal of Quantum Electronics, 18: 1229-1236