How to run PLS Analysis on ERP data - ERP datamat file To run PLS, you need to have a one or more datamats ready, one for each separate group of subjects. All the datamats should have the same selected conditions. Click "Add" button to select a datamat and add it into a group. All the selected datamats will be stacked together rowwise for analysis. The "Number of groups:" field will display how many groups you have added in. If the "Save Datamats" checkbox is selected, the 2D datamat will be saved to a cell array "newdata_lst" in the specified ERPresult.mat file. Each cell represent a group. "Full path" toggles the full path on/off. - PLS Options Mean-Centering PLS option produces results equivalent to the Helmert matrix version described in Lobaugh, et al. (2001). The grand mean across tasks is calculated, and all individual datapoints are subtracted from this value. The newly generated datamat is subjected to singular value decomposition to obtain BrainLVs (Salience), singular values, and other useful results for further analysis. If you run a Behavior PLS, you can may include a Posthoc Data File. This text file contains contrasts across the conditions and/or groups in the analysis, (one contrast per column, one line per condition). This will test for specific differences in brainbehaviour correlations. Most recently, we have developed a "non-rotated" version of Task PLS, wherein a priori contrasts are used to restrict the patterns derived from PLS. - About computation parameters Permutation tests establish the significance of the latent variables. The bootstrap results establish the stability of the LV saliences, and in the case of a BehaviourPLS, the confidence intervals for the brain-behaviour correlations. Typically, the Number of Permutation is usually from 300 to 500 (the higher the better but longer), and the Number of bootstrap is usually set to 100. If you are running Behavior PLS with Number of Bootstrap not equal to zero, you can also choose the Upper Limit of the Confidence Level for the brain- behaviour correlations. The default Confidence Level is set to 95.