Seq-entry ::=
    set {
      class pub-set ,
      descr {
        pub {
          pub {
            medline {
              uid 91288538 ,
              em
                str "1991 Jul 1" ,
              cit {
                title {
                  name "A phorbol ester/diacylglycerol-binding protein encoded
 by the unc-13 gene of Caenorhabditis elegans." } ,
                authors {
                  names
                    str {
                      "Maruyama IN" ,
                      "Brenner S" } } ,
                from
                  journal {
                    title {
                      ml-jta "Proc. Natl. Acad. Sci. U.S.A." } ,
                    imp {
                      date
                        str "1991 Jul 1" ,
                      volume "88" ,
                      issue "13" ,
                      pages "5729-33" } } } ,
              abstract "Mutations in the unc-13 gene cause diverse defects in
 the nervous system of the nematode Caenorhabditis elegans. Molecular cloning
 of the gene and sequencing of the cDNA revealed that the product encodes a
 protein, 1734 amino acids in length, with a central domain with sequence
 similarity to the regulatory region of protein kinase C. The domain was
 expressed in Escherichia coli and shown to bind specifically to a phorbol
 ester in the presence of calcium; diacylglycerol inhibited the binding in a
 competitive manner. These findings confirm that the unc-13 gene product has
 binding sites similar to those of protein kinase C and may be a component of
 an alternative transduction pathway of the diacylglycerol signal to a
 different effector function in the nervous system." ,
              mesh {
                {
                  term "Amino Acid Sequence" } ,
                {
                  term "Animal" } ,
                {
                  term "Base Sequence" } ,
                {
                  term "Caenorhabditis" } ,
                {
                  term "Chromosome Mapping" } ,
                {
                  term "DNA" } ,
                {
                  term "Genes, Structural" } ,
                {
                  term "Molecular Sequence Data" } ,
                {
                  term "Phorbol 12,13-Dibutyrate" } ,
                {
                  term "Protein Kinase C" } ,
                {
                  term "Receptors, Drug" } ,
                {
                  term "Recombinant Proteins" } ,
                {
                  term "Restriction Mapping" } } ,
              substance {
                {
                  type ec ,
                  cit "2.7.1.-" ,
                  name "Protein Kinase C" } ,
                {
                  type cas ,
                  cit "37558-16-0" ,
                  name "Phorbol 12,13-Dibutyrate" } ,
                {
                  type cas ,
                  cit "9007-49-2" ,
                  name "DNA" } } ,
              xref {
                {
                  type genbank ,
                  cit "M62830" } } } } } } ,
      seq-set {
        set {
          class nuc-prot ,
          descr {
            title "[mRNA] unc-13=phorbol ester/diacylglycerol-binding protein
 [Caenorhabditis elegans]; and translated products" ,
            org {
              taxname "Caenorhabditis elegans" } } ,
          seq-set {
            seq {
              id {
                gibbmt 131777 ,
                gibbsq 39237 } ,
              descr {
                mol-type mRNA ,
                title "[mRNA] unc-13=phorbol ester/diacylglycerol-binding
 protein [Caenorhabditis elegans]" ,
                pub {
                  pub {
                    muid 91288538 } ,
                  name "unc-13" ,
                  fig "fig 3" ,
                  num
                    cont {
                       } } } ,
              inst {
                repr raw ,
                mol rna ,
                length 5376 ,
                topology linear ,
                seq-data
                  iupacna "GAATTCATTTTTGAAACAAATCGACCTGACGACGGAATGGTACTCGAATTA
TGGGCCAAAGGGTTTTATGGGACAAATTAATAGGTGTTCATTATATGCCACTTTCGGAGATTAGATACAGCAATGCAG
CAGGTTCGGGTCAATGGCTTCAAATGGATCATGAACTTGAAACAAGAAATGGTCAAACTGTAGGAACACGTGGTCCAA
CAGGTCATAACTTGTTGACTGATGTTCGATTTGAGCTTCCTTTTGATGTACAAGGCCACGACGAAGATGTTCAGTCCA
GATTGCTCGCGTTGAACGGACTCATCGAACACGATCAGCTGGGCCCCAACAACCATCACAGGGCGCCGTTTAACCATT
CGGGTTTGAGTGAAGACAGTGATTATACATCCGACGTGTCCGTTCCAGTCAACCATCATCAATTACATCCAAACAGTT
CGGCACATCAATACGAATCACATCTACATCCACATCGAACACGTCAATTGTTACACACTCGTGAAGGCGCAGCGAGTT
ATGAGGACGAAGAAGACGCCTATCACGCTAGACATCAACCCGAATCCGACGACTATAACCACCAAGACACCTACGATC
AACATTCGTCGTATTATAACGACGAATACGCGCCAAGTGGTAGTAGCAGTCAATATCAACGGCAAGGATATCAAGATC
AAGATCAACAACATCAGAATATATACGAGGACACGGTGACTCCGGTTCGAGAATTTGGCGAGTCGGCCGTTCCGCCGG
CTGCTTCATCGTCTAGACGTCAATTCGATCAGGTTTACGGGTACGCATCTAGCAGTGAAGAACGATATGACACACCGA
TGTCAAGTGGTCGACTGCCAAGAGATGAACCAATTCTTGAGCACAGTGAACCAGAATACGTGTACGATCAGAATGGTT
ATCCCGAAGAAGACAACTATGGAATTAATCCAACATATTCGGAAGATCATTTTGAAGGTCAAACAAATGACTATTCTA
CGACACATCAAGAACCTAATGATTTTAGGAATGATTATAATTCTAGTTATCAAAGAGAATATTGGAATGAGAGTGAGC
CGCTTTCTTATAATAGTAGGCCTCCAAACGGACATATTAGAACAGGTGCAAACACTTGGCGCGAACCATCTACGTCAT
CTCGCCCAACATCTTCCCAAGCATGGAATTATCAAGATGACACTCACCAATATGATGAAGTTGATCGTGGATCCCGAG
TATCTTTCACAAGAACACCATCAGTAGATAGAACAGATAGACCAAGTGAAAGTGGTGGAGGATTTTATGATGAAATGT
CAGAAAGTGGAAGACCAGGGCGTCCAGATTCTCACCATAATTGGAGATATGATTCAATTCAAGAAGAAGATAATGAAA
AAGACAACTGGAAACAACATGTGGAAGGCTACGAAGAAGGACAGGAAGAGAAACAAAAGGACAATCAAAAACCTAATG
ATCATAGTGCAGCTTCACCTCAAGATCACTATCATCGATCAGATTCAACAGCGCAACAAGATTTCGGAAACAATATCG
TTCGTCAGACAATTCAAGAAGAAGAGGAAAAAAGGAATTATCAGGAACTTTGGCATAATGCTTACAAGAGAGTTTGTG
CTGATCTCGGAATTAAGAGTACTGTGCTTGACGGGAACGGGAGTTCGGCTGCCAACGCCTTCTACAAATCAATCGATG
CAGCTCCAAACATGAATGTGGCCCGAACGAAGACAAGTATTCCACTTGTTTCCGAACTTGTTCTAAAGACGATGGCCA
CAAAACGTGCGCAAGCAGGTCTGGCGAATGCGGCAAGAACGACGTTCTCAGATACAGAATTAAAAACTCATGTCTACA
AGAAAACTCTACAAGCATTGATCTACCCGATCAGTGCAACAACTCCTCACAACTTCGCAACTACAACATTCCAAACAC
CCACGTTTTGTTACGAATGCGAAGGATTATTGTGGGGATTGGCTAGACAAGGATTGCGATGTACTCAGTGTCAAGTGA
AAGTTCACGATAAATGTCGTGAATTGCTCAGCGCTGATTGTCTTCAAAGAGCAGCCGAGAAGTCGACGAAACATGGAG
AAGCTGATAGAACACAGTCACTTGTCAATGTTATCAGAGATCGAATGAAGATTCAGGAACAGAATAAGCCTGAAGTGT
TTCAGATGATTAGAACAGTTTTTGATGTGGATGAGAATATTCAAAAAGAGACTTTGAAAACTGTAAAGGCGTCTATTT
TGGAAGGAAGTAGCAAATGGAGTGCAAAGATTACATTGACAGTGCTTTGTGCGCAGGGACTTATTGCCAAGGACAAAA
CCGGAAAAAGTGATCCATATGTGACAGCTCAAGTTGGAAAGACCAAACGAAGGACACGAACCATACACCAAGAGCTCA
ATCCAGTTTGGAATGAGAAATTTCATTTTGAATGCCACAACTCCACGGACAGAATTAAAGTTCGAGTTTGGGACGAGG
ATAATGATTTGAAATCCAAGTTAAGGCAAAAATTGACTCGTGAATCTGATGATTTCTTGGGACAAACAGTAATTGAAG
TTCGAACTTTATCTGGTGAAATGGATGTCTGGTATAATCTTGAAAAGAGAACTGATAAATCTGCTGTATCCGGAGCAA
TTCGATTGCATATCAATGTTGAAATCAAGGGAGAAGAGAAACTTGCACCGTATCATGTTCAGTATACATGTCTTCATG
AACATCTTTTTGCTGCTCATTGTGTAGATGAAGAAGTTAAACTTCCAAAAGTTCGAGGCGAAGATAGTTGGAAAGTTT
GCTTCCAGGAAACTGGTCAAGAGATTGCTGAAGAATTTGCGATGCGGTATGGAATTGAAAGTATTTACCAGGCCATGA
CTCATTTCGCATGCCTATGTACTAGATACATGTGCGCAGGAGTACCTGCAGTCTTGTCAACACTTTTGGCCAATATAA
ATGCGTACTATGCTCATACAACTGCCACGTCAGCAGTCTCTGCTCCTGATAGATTTGCTGCATCCAACTTCGGAAAAG
AACGATTTGTGAAACTTCTTGATCAACTTCACAACAGTTTGAGAATTGACTTGTCAGCATATAGGAATCATTTTCCCT
CTTCGTCGCCTGCCAAACTTCAAGATCTTAAATCAACCGTTGATCTTCTTACTTCAATTACATTCTTTCGCATGAAAG
TTCTCGAGTTGGCGAGCCCACCGCGAGCATCAACAGTTGTTCGCGAATGCGCAAAAGCCTGCATGCAGCAGACCTATC
AACTGATGTTTGAATCATGCGCTGAACAATTCCCAATTTTGGACACTTCAGTTCAATTCTGGTATGAATTCATCGACT
ATATAATGCGAGTAATTGAAGAAGATCAAAAAAACTATACACCAGCATTGAATCAATTCCCTCAAGAACTGAATGTTG
GGAATTTATCCGCTGAAACACTTTGGAGTATGTACAAGAATGATTTGAAAATGGCATTAGAAGAACATGCACAAAAGA
AACGATGCAAGACGCCTGAATATATGAATTTATATTTCAAGGTTAAAGGTTTCTATTTCAAATATGTCGCTGATCTTT
CAACATACAAATCATCAATTCCCGAGTTTCCTGCATGGTTTATTCCATTCGTCATGGATTGGCTCAATGAAAATGACG
AACATTCGATGGATATTCTCAGAAATGCATACAACTGTAGACAAGCTGACACTTCCCACAAACATCAGAACACACAAA
TTCTCGAACATGTTGTTGATGTGTTCACCCAACTAAACGCTGCATTGAAGCTCCTCAAACAAATGGACTGTCCAAATC
CAGAAGTTGCTGCTGATATGATGAAGAGATTCAGTAAAACATTGAATAAAGTTCTTCTTGCATACGCTGATATGGTTC
AAAAAGATTTTCCGAAATTTGCACATGACGAGAAGCTTGCGTGTATTCTAATGAACAATGTTCAGCAATTACGTGTGC
AACTCGAAAAGATCTACGAAACAATGGGCGGAGCAGAACTTGACGAACATATTGGACAAGTGCTTACAGTCCTTCAAA
AGAAGTTGAATTCTGTTCTAGACAGACTTTCAGCTGAATTTGTGACCACTTTGGAACCCCATATTCATGAACAAACAA
TCAAATTGGGTATGCTCCTCGTTAAAATAAAGGGTCCACAACTTCAAAAGACACAAGTTCAACCGGAAGCCGATGCAG
TTCTAGAACCGTTGATGGATCTTCTTGAAGGATCATTGAGAAGATACGCGGATCAGTGTGAGAAAACGGTTCTGAAGT
ATATTCTCAAAGAACTTTGGAAAATAACAATTGTTAATATGGAAAAGAGAGTTGTTCTACCACCATTATCAGATAAGG
CTCTATTGAAACAACTGCCGAATGCCAAAATTGGAGATGTTACGAAGTTAATGTCAACAAACAATCAAAGTATAAAGG
GAATGAATTCCGTTAAAGATATGATGGATATGGCAAGAGAATCTGAAAAATCTCTAACACCCAGACAATGTACAGTTC
TTGATTGTGCACTTGACGCCATTAAAGACAGTTTCCATGCATCCGGAAAAGGACTTAAGAAATCATTCTTTGAGAAAT
CACCTGAACTCCAATCTCTCAAATATGCACTCAGTTTATATACACAGACTACTGAGCAACTCATCAAAACGTTTATTA
CAAGCCAGCGACAGCAAGATCTCCCAAGTCAAGAGCAACCAGTTGGAGAAGTTAGTGTTCAAGTTGACTTATTCAGTC
ATCCAGGCACTGGGGAACAGAAGGCTACCGTTAAAATCCTTGCCGCAAACGATCTTCGATGGCAAACCTCGTCAGCTT
TCAAACCATTTGTTGAAGTGCATCTTGTCGGACCACATCTTTCAGACAAAAAGAGAAAATGGTCGACAAAGACCAAAG
CAGGCAACTGGGCTCCAAAATTCAACGAAACTTTTCATTTCTTCCTTGGAAATGAAGGAGAACCTGAACATTACGAGC
TTATGTTTCAAGTCAAGGATTACTGTTTTGCACGAGATGATCGAGTTGTTGGAGTTGGAGTTCTGCAGTTATCATCTG
TCGTTGATCAAGCGGGATCCTGCGCCATGTGGGTTCAACTTGGAACTCGACTGCATATCGACGAGACTGGGCTCATTT
TGCTCAGGATTCTTTCACAGCGTCAAACTGATGAGGTGGCAAAGGACTTTGTACGCCTAAAAACGGAATGCCGATACG
AGACAGAAACAGTAATGGCTGCATCAGCATCAAGTCAAAACATCAATCGAACATAGTGATTCAAGTGCCTTTTTTTGT
TGGATTCAAAGCAATAGAGTTGTTCTTCATTCGATCAATTTTCATCTCCTCTACGAATACCTGTACATTTTGTCGTTT
TTTTTTTCTTTCCTCGAATTC" } ,
              annot {
                {
                  data
                    ftable {
                      {
                        id
                          gibb 131780 ,
                        data
                          gene {
                            locus "unc-13" ,
                            desc "phorbol ester/diacylglycerol-binding protein" } ,
                        location
                          packed-int {
                            {
                              from 0 ,
                              to 5375 ,
                              strand plus ,
                              id
                                gibbsq 39237 } } } } } } } ,
            seq {
              id {
                gibbmt 131799 ,
                gibbsq 39239 } ,
              descr {
                mol-type peptide ,
				method both ,
                title "[Peptide] phorbol ester/diacylglycerol-binding protein
 [Caenorhabditis elegans]" ,
                pub {
                  pub {
                    muid 91288538 } ,
                  name "unc-13" ,
                  fig "fig 3" ,
                  num
                    cont {
                       } } } ,
              inst {
                repr raw ,
                mol aa ,
                length 1734 ,
                topology linear ,
                seq-data
                  iupacaa "MGQRVLWDKLIGVHYMPLSEIRYSNAAGSGQWLQMDHELETRNGQTVGTRG
PTGHNLLTDVRFELPFDVQGHDEDVQSRLLALNGLIEHDQLGPNNHHRAPFNHSGLSEDSDYTSDVSVPVNHHQLHPN
SSAHQYESHLHPHRTRQLLHTREGAASYEDEEDAYHARHQPESDDYNHQDTYDQHSSYYNDEYAPSGSSSQYQRQGYQ
DQDQQHQNIYEDTVTPVREFGESAVPPAASSSRRQFDQVYGYASSSEERYDTPMSSGRLPRDEPILEHSEPEYVYDQN
GYPEEDNYGINPTYSEDHFEGQTNDYSTTHQEPNDFRNDYNSSYQREYWNESEPLSYNSRPPNGHIRTGANTWREPST
SSRPTSSQAWNYQDDTHQYDEVDRGSRVSFTRTPSVDRTDRPSESGGGFYDEMSESGRPGRPDSHHNWRYDSIQEEDN
EKDNWKQHVEGYEEGQEEKQKDNQKPNDHSAASPQDHYHRSDSTAQQDFGNNIVRQTIQEEEEKRNYQELWHNAYKRV
CADLGIKSTVLDGNGSSAANAFYKSIDAAPNMNVARTKTSIPLVSELVLKTMATKRAQAGLANAARTTFSDTELKTHV
YKKTLQALIYPISATTPHNFATTTFQTPTFCYECEGLLWGLARQGLRCTQCQVKVHDKCRELLSADCLQRAAEKSTKH
GEADRTQSLVNVIRDRMKIQEQNKPEVFQMIRTVFDVDENIQKETLKTVKASILEGSSKWSAKITLTVLCAQGLIAKD
KTGKSDPYVTAQVGKTKRRTRTIHQELNPVWNEKFHFECHNSTDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTVI
EVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHINVEIKGEEKLAPYHVQYTCLHEHLFAAHCVDEEVKLPKVRGEDSWK
VCFQETGQEIAEEFAMRYGIESIYQAMTHFACLCTRYMCAGVPAVLSTLLANINAYYAHTTATSAVSAPDRFAASNFG
KERFVKLLDQLHNSLRIDLSAYRNHFPSSSPAKLQDLKSTVDLLTSITFFRMKVLELASPPRASTVVRECAKACMQQT
YQLMFESCAEQFPILDTSVQFWYEFIDYIMRVIEEDQKNYTPALNQFPQELNVGNLSAETLWSMYKNDLKMALEEHAQ
KKRCKTPEYMNLYFKVKGFYFKYVADLSTYKSSIPEFPAWFIPFVMDWLNENDEHSMDILRNAYNCRQADTSHKHQNT
QILEHVVDVFTQLNAALKLLKQMDCPNPEVAADMMKRFSKTLNKVLLAYADMVQKDFPKFAHDEKLACILMNNVQQLR
VQLEKIYETMGGAELDEHIGQVLTVLQKKLNSVLDRLSAEFVTTLEPHIHEQTIKLGMLLVKIKGPQLQKTQVQPEAD
AVLEPLMDLLEGSLRRYADQCEKTVLKYILKELWKITIVNMEKRVVLPPLSDKALLKQLPNAKIGDVTKLMSTNNQSI
KGMNSVKDMMDMARESEKSLTPRQCTVLDCALDAIKDSFHASGKGLKKSFFEKSPELQSLKYALSLYTQTTEQLIKTF
ITSQRQQDLPSQEQPVGEVSVQVDLFSHPGTGEQKATVKILAANDLRWQTSSAFKPFVEVHLVGPHLSDKKRKWSTKT
KAGNWAPKFNETFHFFLGNEGEPEHYELMFQVKDYCFARDDRVVGVGVLQLSSVVDQAGSCAMWVQLGTRLHIDETGL
ILLRILSQRQTDEVAKDFVRLKTECRYETETVMAASASSQNINRT" } ,
              annot {
                {
                  data
                    ftable {
                      {
                        id
                          gibb 131801 ,
                        data
                          prot {
                            desc "phorbol ester/diacylglycerol-binding protein" } ,
                        location
                          packed-int {
                            {
                              from 0 ,
                              to 1733 ,
                              strand plus ,
                              id
                                gibbsq 39239 } } } } } } } } ,
          annot {
            {
              data
                ftable {
                  {
                    id
                      gibb 131779 ,
                    data
                      cdregion {
                        frame one ,
                        code {
                          id 1 } } ,
                    product
                      whole
                        gibbsq 39239 ,
                    location
                      packed-int {
                        {
                          from 50 ,
                          to 5251 ,
                          strand plus ,
                          id
                            gibbsq 39237 } } } } } } } } }
